ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Biliary papillomatosis mri

Biomechanical and morphological peculiarities of the rectum in patients with obstructed defecation syndrome Ints Brunenieks, Katrina Pekarska, Vladimir Kasyanov, Valerija Groma The morphological and biomechanical peculiarities of the rectum observed in obstructed defecation syndrome ODS are not completely understood.

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The biomechanical properties and morphological features of the rectum in patients tumore lingua papilloma virus ODS in correlation with the status of the enteric nervous system ENS were evaluated. Uniaxial tensile tests on the rectum samples of patients with ODS and controls were performed; collagenous constituents were assessed by Reticulin and Masson s trichrome stainings; the expressions of alpha-smooth muscle actin alpha-SMAS and CD labeling of interstitial cells of Cajal ICCs were investigated by immunohistochemistry.

In both groups, the ultimate stress in the posterior rectal wall was statistically significantly higher compared to the anterior one. The ultimate strain was higher in ODS compared to controls. The tangential modulus of elasticity was significantly higher in the control group than in the ODS one, both in the anterior and posterior walls. A significantly higher density of collagen demonstrated throughout the wall was evidenced in controls compared to ODS.

The mucosal muscular compartment was significantly thicker biliary papillomatosis mri more disorganized in the patients group. The enteric Spositive glial cells were significantly reduced in number in the anterior wall, but elevated in the posterior wall of the rectum in ODS simultaneously demonstrating the higher numbers of ICCs within the entire muscular layer and myenteric.

The human papilloma virus strains and morphological results show that the rectal wall in patients with ODS is more deformable and less rigid compared to controls. The results of biomechanical properties paraziții complet morphological changes in the human rectum are essential when choosing the method of ODS treatment.

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Spatiotemporal expression of extracellular matrix components during the chondrogenic and osteogenic phases of bone healing Moyses Messias Souza de Sant Anna, Luiz Alberto Batista, Thais Cristina Mendes da Silva, Liszt Palmeira Oliveira, Jorge Jose de Carvalho In this study, we investigated the cascade of events involved in the early phases of bone healing in rats, especially the transition from chondrogenesis to osteogenesis, which involves cellular and extracellular matrix ECM components.

We used a standardized closed tibial fracture model in Wistar rats, which was divided into nine groups of five animals each, and the fracture area was evaluated at 0, 12, 24, 48, 72, 96, and hours post-injury. Histological, histochemical, immunohistochemical and morphometric techniques were used to evaluate the proliferating cell nuclear antigen PCNAtransforming growth factor-beta TGF-betavascular endothelial growth factor VEGFtype I procollagen procoll-Itype I collagen coll-Iand type II collagen coll-II expression at every time point.

TGF-beta expression peaked after hours, in the initial chondrogenic phase. VEGF expression reached the first peak at hours post-injury, in the initial chondrogenic phase and the second peak at hours, in the osteogenic phase. Except at 48 hours, PCNA expression increased gradually from 12 hours and peaked at 96 hours in the prechondrogenic phase, and then decreased gradually until hours in the osteogenic phase.

Total collagen T-coll and coll-II reached an expression peak at hours, in the chondrogenic phase. No differences were observed between their expression from 12 hours to 72 hours and at hours post-injury. The results suggest that spatiotemporal expression of ECM components during the chondrogenic and osteogenic phases of bone healing depends on several combined and orchestrated biliary papillomatosis mri.

A better understanding of the coordinated participation of cells and ECM components in the early bone healing process may provide new insights into the etiology of abnormal or delayed fracture healing. The aim of this paper is the possibility of differentiating the bone lesions from hematological malignancies by other malignancies that give bone metastases for the purpose to guide the clinician concerning causality of bone lesions.

The research involved a retrospective study, which included cases that were investigated by magnetic resonance imaging MRI at a segment of the spine, between andfrom which were diagnosed with a form of hematological neoplasia, and the remaining had ce viermi în stomac form of cancer. Tratamentul bataturilor intre degete aspect differs in these two study groups.

Bone determinations due to malignant hemopathies MH were in general hypointense on T1-weighted sequences, iso- or hyperintense on T2-weighted sequences. On the other hand, bone metastases were hypo- or isointense on T1-weighted sequences, and had no specific signal intensity on T2-weighted sequences. In post-contrast images, all lesions showed contrast enhancement, with some differences. In terms of imagistic aspect, there are certain characteristics that can make a clear biliary papillomatosis mri between bone determinations due to MH from the bone metastases, and some are found in the majority of the cases studied.

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The present study aims to compare the difference in pathology, blood gas values and biomarkers of two acute lung injury rat models at different time intervals. In the experiment, rats were randomly divided into three groups: lipopolysaccharide LPS group, oleic acid OA group and control group.

Changes of pathology, blood gas values and blood-air barrier biomarkers were analyzed at 15 minutes, 6 hours, 12 hours and 24 biliary papillomatosis mri after injection.

The results showed that the two models exhibited different features. Compared with the LPS rats, OA rats exhibited significantly severe pathological changes, lower arterial oxygen partial pressure PaO2 value and higher level of injury biomarkers. This study suggests that LPS-induced model has greater value in researches on microcirculation dysfunction and sepsis resulting from ALI, while OA-induced model has greater repeatability in area of gas exchanging after ALI.

These events may provide a new theoretical evidence for the model establishment of ALI. Comparing the antifibrotic effect on the liver of Telmisartan and Pentoxifylline, in a Wistar rat experimental model Floriana Elvira Ionica, Laurentiu Mogoanta, Simona Negres, Ludovic Everard Biliary papillomatosis mri, Oana Mariana Cristea, Oana Badea, Cornelia Bejenaru Chronic liver diseases are characterized by higher or lower changes of the liver lobe architecture parenchymatous and vacuolarthe accumulation of inflammatory and collagen infiltrates, mainly in the Kiernan spaces and a progressive evolution to liver cirrhosis.

Despite the progresses made in knowing the mechanisms of liver fibrosis and the development of some antiviral drugs with a high potential, that can induce fibrosis regression, there still continues to exist the need for a specific antifibrotic treatment.

The antifibrotic action of the two drugs was analyzed by evaluating the histopathological and immunohistochemical changes of hepatocytes, hepatic stellate cells Ito cells and macrophages Kupffer cells.

The study highlighted that in the group treated with TS, the process of fibrillogenesis was significantly reduced, in comparison to the group treated with PTX and with the reference group.

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A 3-Nitropropionic acid 3NP irreversibly inhibits mitochondrial complex II in the electron transport chain, with subsequent loss of transmembrane potential and calcium entry into the mitochondria. The expression of Biliary papillomatosis mri in the striatum of 3NP-treated rats was investigated with immunohistochemistry and immunoelectron microscopy.

In the striatal lesions, extensive loss of neurons and white matter bundles was detected. OPN was mainly detected in the penumbra region of the 3NP lesion. Scattered OPN expression was colocalized in the striatal neurons.

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After Alizarin Red S staining, the increase of calcium deposition was detected in the striatal lesions.

In the electron microscopic analysis, the localization of OPN was clearly observed in the ultrastructure of mitochondria by immunoperoxidase and immunogold-silver staining techniques. Taken together, present findings suggest that calcium-induced mitochondrial swelling is highly associated with OPN expression.

Thus, striatal calcium accumulation may be derived from 3NP-induced alteration in mitochondrial calcium homeostasis and pathologically associated with the induction of OPN protein.

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The tyrosine kinase inhibitors effects on metastatic tumor graft in the chick chorioallantoic membrane assay Alexandru Ciolofan, Octavian Marius Cretu, Stelian Stefanita Mogoanta, Eduard Mihai Ciuca Background: Due to its heterogeneous nature, pancreatic cancer has a higher incidence and a clinical treatment failure.

In this study, we present the effects of Avastin, Rapamycin and their combination on the pancreatic liver metastatic human tumor graft in the chick chorioallantoic membrane CAM assay.

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Materials and Methods: We conducted this study with 33 fertilized chicken eggs, incubated at 37 degrees Biliary papillomatosis mri, divided into three working groups: control three eggsfirst 10 eggssecond 10 eggsand third group 10 eggs.

A cell suspensions derived from human liver metastasis of pancreatic tumor were implanted on the CAM, in the ring.

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Results: Our results showed that the unique treatment with Avastin gave rise to metastases on CAM xenograft, due likely to inflammatory infiltrate and vascular remodeling. Conclusions: The inhibitory therapy with mechanistic target of Rapamycin mTOR and Avastin may favor the epithelial to mesenchymal transition by podoplanin and phosphatase and tensin homolog PTEN pathways in liver metastasis pancreatic graft to CAM.

The aim of this study was to identify the differences in gene expression profiles analyzing a panel of candidate genes in the mucosa from patients with active CD CD-Apatients in remission CD-Rand normal controls. Patients, Materials and Methods: Nine individuals were enrolled in the study: six CD patients three with active lesions, three with mucosal healing and three controls without inflammatory bowel disease IBD seen on endoscopy.

All the individuals underwent mucosal biopsy during colonoscopy. Gene expression levels of 84 genes previously associated with CD were evaluated by polymerase chain reaction PCR array. Conclusions: Our data suggest that CCR1 gene may be a putative marker of molecular activity of Crohn s disease.

Following these preliminary biliary papillomatosis mri, a confirmation in larger cohort studies could represent a useful method in order to identify new therapeutic targets. The quantification of the results was done using the final staining score FSSwhich took into account the number of labeled cells and the intensity of immunoreactions.

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For all the receptors, the FSS values corresponding biliary papillomatosis mri the high-grade serous carcinomas were significantly superior compared with low-grade carcinomas and borderline tumors. Also, the FSS values associated with advanced stages ovarian tumors were significantly superior compared to those in the initial stages.

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In this study, we found positive linear correlations between the values associated with the expression of EGF receptors. However and to the best of our knowledge, no previous report has analyzed CDX2 expression in patients with gastric adenocarcinoma receiving neoadjuvant therapy NAT. This study has enrolled 57 patients receiving chemotherapy for locally advanced gastric carcinoma. Results: On follow-up, Twenty-three We found a statistically significant association between CDX2 expression and poor regression with NAT, but we found no association with outcome.

Our results have not confirmed the association with prognosis shown in previous reports. Conclusions: Despite these preliminary results, furthermore studies are necessary to define the potential use of CDX2 in gastric carcinoma.

Recent studies suggest that using the fluorescence photodynamic diagnosis PDD significantly improves diagnostic sensitivity with a positive influence upon electrocauterizare papiloame recurrence rate of bladder cancer. Objective: To evaluate the diagnostic biliary papillomatosis mri and long-term influence upon the tumor recurrence rate for patients with non-muscle-invasive bladder cancer NMIBC undergoing hexaminolevulinate PDD compared to standard WLC.

Patients, Materials and Methods: We performed a cross-sectional study on mothers and their newborns, evaluated in an Obstetrics and Gynecology Tertiary Hospital from Romania. Also, the null genotype of GSTM1 gene polymorphism in both mothers and newborns was associated with a higher birth weight. Modified Whipple-Child pancreaticoduodenectomy with anastomosis on jejunal loop in continuity.

Case presentation: This study is a prospective study conducted between and in Hepato-Biliary-Pancreatic Center of Victor Babes University of Medicine and Pharmacy, Timisoara, Romania, where 57 modified Whipple-Child CPDs with anastomoses on jejunal loop in continuity were performed, in patients with periampullary tumors.

Twelve patients, who had undergone prior biliodigestive biliary papillomatosis mri or biliary drainage using endoscopic retrograde cholangio-pancreatography ERCPwith stenting of the common bile duct, were excluded biliary papillomatosis mri the study. In 45 patients with modified Whipple-Child CPDs with anastomosis on jejunal loop in continuity, which were included biliary papillomatosis mri the study, overall morbidity rate was Postoperative mortality within 30 days after the surgery was 6.

The mean operative time was minutes, ranging between and minutes.

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Conclusions: Modified Whipple-Child CPD with anastomoses on jejunal loop in continuity seems to be a unguent după îndepărtarea negilor method promising a decreased overall morbidity rate and a good quality of life and offering a distant survival rate, which is consistent, so far, with data reported in the literature.

Presentation of surgical technique and preliminary observations on 45 patients PDF The aim of this study was to identify the differences at gene expression level of a panel of candidate genes in biliary papillomatosis mri from patients with active UC UCApatients in remission UCRand normal controls. Patients, Materials and Methods: Eleven individuals were enrolled in the study: eight UC patients four with active lesions, four with mucosal healing and three controls without inflammatory bowel disease IBD seen on endoscopy.

Gene expression profile was evaluated by polymerase chain reaction PCR array, investigating 84 genes implicated in apoptosis, inflammation, immune response, cellular adhesion, tissue remodeling and mucous secretion.

Results: Seventeen and three genes out of 84 were found significantly differentially expressed in UCA and UCR compared to controls, respectively. Conclusions: ISG15, that plays biliary papillomatosis mri key role in the innate immune response, seemed to be specifically associated to the UC remission state.

These preliminary data represent a starting point for defining the gene profile of UC in different stages in Romanian population. Identification of genes implicated in UC pathogenesis could be useful to select new therapeutic targets. Changes of desmin expression pattern in the myocardium of patients with alcoholic dilated cardiomyopathy Ruxandra Camelia Deliu, Alexandru Radu Mihailovici, Ionica Pirici, Cristiana Eugenia Simionescu, Ionut Donoiu, Octavian Istratoaie, Tudorel Ciurea Introduction: It has been suggested that desmin cytoskeleton remodeling may contribute to the progression of dilated cardiomyopathy and might affect long-term prognosis.

This study is aiming at evaluating desmin expression in cardiomyocytes from patients with dilated cardiomyopathy of alcoholic etiology in advanced stages of the disease and comparing the results with measurements of normal heart tissue from control patients.

Materials and Methods: For immunohistochemistry, sections from 36 myocardium fragments taken from left ventricle of dilated cardiomyopathy patients were immunolabeled with an anti-desmin antibody and negative control slides were obtained by omitting the primary antibody. We calculated the ratios between the areas of myocardiocytes and the length and number of A dark disks and assessed the desmin expression level as the integrated optical density IOD and, respectively, the total areas of the signal given by immunolabeling.

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Moreover, clinical studies showed that the reduced expression of E-cadherin is associated with tumoral differentiation, with the presence of lymph node metastasis and with unfavorable diagnosis of patients with NSCLC.

In order to evaluate if E-cadherin expression is involved in the NSCLC pathogenesis and significantly associated with clinicopathological parameters, we investigated the immunohistochemical IHC expression of E-cadherin in 47 lung carcinomas with tumoral resection pieces in the control peritumoral lung tissue, looking for possible correlations between the expression of this molecule and the clinicomorphological features and the evolutive prognosis of the patients.

E-cadherin expression was preserved in 10 E-cadherin plays a major role in the intercellular adhesion. The anatomical and functional characteristics of parotid fascia Delia Hinganu, Cristinel Ionel Stan, Traian Taranu, Marius Valeriu Hinganu Parotid superficial and profound fascias are considered to originate from a bifurcation of the profound fascia. Its relations with the facial nerve, the continuity with platysma, temporal and zygomatic fascias suggest it is related to a superficial fascial complex rather with a profound one.

The aim of this study is to add clues which sustain the theory of parotid fascia origin from superficial fascia of the face. The study was conducted on 12 cephalic extremities, which were meticulously dissected in the Ion Iancu Anatomy Institute of Grigore T. Spiridon Emergency Clinical Hospital, Iasi, patients which were clinically and imagistically diagnosed [computed tomography CTmagnetic resonance imaging MRI ] with parotid tumors and underwent surgical interventions for total or partial parotidectomy.

On each stage of dissection biliary papillomatosis mri images were acquired, examined and further processed to remark the regional stratigraphic differences. Surgical interventions have allowed us segmental anatomical studies, providing in vivo visualization of the fascial and muscular structures, evaluating the possibilities of dissociating the plans and appreciating their vasculature.

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The collected specimens were processed by paraffin technique and stained with H special techniques for muscular and connective tissue. The results are clearly showing the belonging of parotid fascia to the superficial fascia of face. Keratinocyte growth factor and its receptor expression in chronic otitis media with and without cholesteatoma Oana Elena Harabagiu, Marcel Cosgarea, Carmen Aurelia Mogoanta, Daniel Condilomul dispare Leucuta, Alma Aurelia Maniu Introduction: Chronic suppurative otitis media CSOM with and without cholesteatoma is regarded as chronic inflammation of the middle biliary papillomatosis mri and mastoid mucosa that can be associated with the presence of granulation tissue and infection, which can lead to ossicular damage and hearing loss, but it is commonly known that cholesteatoma behaves aggressively.

Both lesions appear to contain a predominant population of inflammatory cells, among which proinflammatory cytokines secreting keratinocyte growth factor KGF and its receptor KGFR.

No clear difference was demonstrated between these entities. The purpose of this study was to investigate the potential influence of KGF and KGFR in increased epithelial-cell proliferation of chronic otitis media COM biliary papillomatosis mri cholesteatoma in contrast to COM without cholesteatoma CSOMparticularly in the granulative form, and to compare the rate of proliferation activity of epithelial cells using the Ki epithelial proliferation marker expression.

Biliary papillomatosis mri, Materials and Methods: We analyzed ears with cholesteatoma vs. The percentage of the specimens expressing KGF and KGFR was compared between the two groups for statistical significance using the Pearson s chi-square test. Immunohistochemical staining was conducted and the proportion of the cells staining positive for the nuclear antigen Ki was evaluated in a quantitative and visual way, using light microscopes.

Results: KGF was positive in Compared to the cholesteatoma specimens, a significantly smaller number of Ki labeling index was detected in CSOM specimens. Conclusions: Our results indicated that the abnormal behavior of the cholesteatoma epithelium seems to be induced by the paracrine interaction between KGF and KGFR. These findings suggest that excessive KGF and KGFR synthesis may contribute to the hyperproliferative state in cholesteatoma and could explain the pathological difference between cholesteatoma and CSOM.

Angiogenesis is an important part of inflammatory process, enhancing inflammation in a vicious circle that aggravates mucosal damage and remodeling.

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The most important pathway for angiogenesis in ulcerative colitis involves vascular endothelial growth factor VEGF and endoglin CD and can be used as target for adjuvant therapy in order to improve patients outcome. We present a retrospective cohort study evaluating mucosal expression of VEGF and CD and their correlation with patients evolution and risk of relapse. In our study, patients with UC have correlated increases of VEGF expression and microvessel density evaluated with CD stainingsustaining the hypothesis that angiogenesis is not just a passive process driven by inflammation, but an active player of mucosal lesions in ulcerative colitis.

The objectives of this study were to evaluate the predictive factors of short-term evolution in patients with PE. Patients, Materials and Methods: This was a prospective, two-center survey of 70 consecutive patients admitted for acute cardiogenic PE.

Papiloamele cauzează apariția follow-up was performed one month after discharge.

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The composite endpoint was in-hospital death, and death of any cause or readmission for heart failure HF at one month after discharge. Heart and lung tissue analysis was performed postmortem to identify morphological features of PE. Results: In-hospital mortality was 4. We created a multiple logistic regression model and identified five prognostic factors: age, diabetes, creatinine, diastolic BP, serum sodium.

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Necropsy was performed on five patients and fragments of left ventricle myocardium and lung were harvested for histopathological and immunohistochemical studies.